Part:BBa_M36689
HSE 2x Promoter + Bxb1 Recombinase w/ NLS + SV40 Terminator + attB site + CMV Reverse + attP site
This part incorporates a HSE 2x optimized promoter for a highly elucidated heat shock transcription factor. Once this site has been activated, it signals a Bxb1 Recombinase, a serine based recombinase. When used as an invertase, Bxb1 identifies attB and attP sites, cuts at these sites, and inverts the DNA sequence in between these two markers. Bb1 integrase is capable of performing this process without additional cofactors, which has been confirmed by in vitro studies. The attB and attP sites are both incorporated into this composite with a CMV reverse complement promoter within. This reverse promoter will, as a result, be switched "on" once activated by the HSE 2x transcription factor site and may be used to activated some sort of sensor.
Sequence and Features
- 10INCOMPATIBLE WITH RFC[10]Illegal XbaI site found at 12
Illegal XbaI site found at 31 - 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 77
Illegal NheI site found at 296 - 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 570
Illegal XhoI site found at 657 - 23INCOMPATIBLE WITH RFC[23]Illegal XbaI site found at 12
Illegal XbaI site found at 31 - 25INCOMPATIBLE WITH RFC[25]Illegal XbaI site found at 12
Illegal XbaI site found at 31
Illegal NgoMIV site found at 1209
Illegal NgoMIV site found at 1296
Illegal AgeI site found at 346 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 1404
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