Composite

Part:BBa_M36689

Designed by: Shankara Anand   Group: Stanford BIOE44 - S11   (2014-10-24)


HSE 2x Promoter + Bxb1 Recombinase w/ NLS + SV40 Terminator + attB site + CMV Reverse + attP site

This part incorporates a HSE 2x optimized promoter for a highly elucidated heat shock transcription factor. Once this site has been activated, it signals a Bxb1 Recombinase, a serine based recombinase. When used as an invertase, Bxb1 identifies attB and attP sites, cuts at these sites, and inverts the DNA sequence in between these two markers. Bb1 integrase is capable of performing this process without additional cofactors, which has been confirmed by in vitro studies. The attB and attP sites are both incorporated into this composite with a CMV reverse complement promoter within. This reverse promoter will, as a result, be switched "on" once activated by the HSE 2x transcription factor site and may be used to activated some sort of sensor.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 12
    Illegal XbaI site found at 31
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 77
    Illegal NheI site found at 296
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 570
    Illegal XhoI site found at 657
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 12
    Illegal XbaI site found at 31
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 12
    Illegal XbaI site found at 31
    Illegal NgoMIV site found at 1209
    Illegal NgoMIV site found at 1296
    Illegal AgeI site found at 346
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1404


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Categories
Parameters
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